Hi,
Are your "diameter background circle (px)" in the Extract job and
"Mask diameter (A)" in Class2D more-or-less the same?
Note that the unit is different.
Best regards,
Takanori Nakane
> We are doing some preliminary neg stain analysis of a new project and after
> picking and extracting particles, Class2D is complaining that our images
hav
> en’t been normalised to average background = 0 and stddev = 1. The classes
> we are getting after this run are not great.
>
> The ‘Normalise Particles’ box was checked as Yes in the Extract job so th
> is is a little confusing.
>
> Is there something different we should be doing for neg stain images ?
>
> Laurence
>
> --------------------------------------------------------------------------------
> Laurence H. Pearl PhD FRS FMedSci
>
> Professor of Structural Biology
> Genome Damage and Stability Centre
> School of Life Sciences, University of Sussex
> Brighton, BN1 9RQ, UK
>
> +44-(0)1273 678349
> --------------------------------------------------------------------------------
> "Live Modestly and do Serious Things .. "
> - Dorothy Crowfoot Hodgkin
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