Dear Takanori,
The particles have been properly normalised. The diameter I've specified in the Class2D job is 120Å. Pixel size of the not-downscaled micrographs was 1.09Å and the background diameter I used in the Extract job I ran before the 2D class was 225 pixels (with a particle box size of 300). I've also downscaled particles to 80 pixels, getting a new pixel size of 4.0875 Å/pixels.
Am I missing something?
All the best,
Leonardo
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