Dear all,
after doing particle polishing, which looks pretty normal, I did a classification in 2D (30 classes with ignore first peak option) or in 3D (4 classes, full ctf) with the polished particles.
The result is, that all particles end up in a single class with about 95 % of the particles while the other classes are basically empty.
This happens especially in 3D, while in 2D this effect is only seen in the first 15 iterations.
However, the single class looks good, but the other classes are somewhat grainy.
Without polishing this does not happen and the distribution of particles is rather even.
Has anyone else experienced this effect?
Or does anyone know what could be the reason for it?
I have seen this behavior before, even with different proteins / samples / datasets.
Thank you very much,
Thomas
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