... although this raises a question: if there are no free reflections,
then why is a "Rfree" score being reported at all?
On 2019-08-30 16:53, Christian Roth wrote:
> No. As pointed out by Pavel, there are no reflections flagged as
> "free reflections" used to calculate Rfree. Therefore you don't get a
> real Rfree. No model bias issue etc. If you have a Rfree using ccp4
> programs, than you should used that particular original mtz in phenix.
> It should detect the flagged "free reflections" automatically.
>
> Cheers
> Christian
>
> On Fri, Aug 30, 2019 at 5:24 PM Tung Thanh Dinh <[log in to unmask]>
> wrote:
>
>> I have been using elipsoidal truncated data all long. And at first i
>> couldn't find a well homologous model (50% homology is the best i
>> can find) so I use Mr Bump and Buccaneer to create a model to phase
>> and autobuild. After a lot of refinement I obtained "good" model
>> with good statistics. Then I used this "good" model to either phase
>> and autobuild again. So this may be due to the model bias?
>>
>> Kindest regards,
>> Tung Dinh
>>
>> PhD student
>> The University of Georgia
>> Department of Chemistry
>> -------------------------
>>
>> FROM: CCP4 bulletin board <[log in to unmask]> on behalf of Tung
>> Thanh Dinh <[log in to unmask]>
>> SENT: Friday, August 30, 2019 9:36:58 AM
>> TO: [log in to unmask] <[log in to unmask]>
>> SUBJECT: [ccp4bb] I am doing phenix refinement now. Is it a problem
>> if the r-work and r-free values are equal?
>>
>> After phasing with phenix, i clicked on the phenix.refine ribbon.
>> Since then for every macrocycle of refinement, r-work and r-free
>> values are always the same. Is this a problem that I need to fix?
>>
>> Kindest regards,
>> Tung Dinh
>>
>> PhD student
>> The University of Georgia
>> Department of Chemistry
>>
>> -------------------------
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