Thank you so much Takanori. I’ll try your suggestions to run Class2D. Not sure how the defective pixels look like on 2D average.
By the way, a new issue related to MultiBody refinement:
Error:
Array by array:different shapes (*)
I ran MultiBody successfully on a different job. There’s nearly no change of settings for this error job.
Best,
Q
> On Jul 22, 2019, at 2:44 PM, Takanori Nakane <[log in to unmask]> wrote:
>
> Hi,
>
> This is caused by detector defects.
>
> Polishing does not take hot pixels and defect information into account. Even when they were concealed during initial motion correction,
> they can come back after Polishing.
>
> Please perform Class2D on shiny particles to remove particles with
> defective pixels. In RELION 3.1, Polishing will take care of hot
> pixels and defects.
>
> Best regards,
>
> Takanori Nakane
>
> On 2019/07/22 21:54, qianhui qu wrote:
>> Hi there,
>> This is the first time I had such an interesting result with Polished data, given that I’ve run Polish several times without any issue.
>> Any thoughts?
>> Thanks.
>> Q
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