Hello,
I'm wondering how to take a group of particles with assigned Euler angles and have Relion generate two half maps from the particles without any further rotation, shifting, or alignment. I'm not sure what the best Relion command and additional flags for this job would be. Help anyone can share would be greatly appreciated.
Just for more information on my exact situation: I have done a 3D refinement on signal subtracted particles and have reverted the particles back to non-signal subtracted versions. To my knowledge Relion has now created a original.star file with the list of the non-signal subtracted particles and the Euler angles generated during the signal subtracted refinement. I would simply like to reconstruct 2 half maps from these particles using the corresponding Euler angles in order to generate a final sharpened map to compare to the one generated from the signal subtracted particles.
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