Dear Faisal,
To use LibG in the CCP-EM Refmac interface please click on the 'Refinement options' button and then switch the 'Nucleic acids' option to true. This will generate and apply the LibG restraints for you.
If you want to only use the restraints at specific locations you can define the range in the 'Nucleotide range' box otherwise they will be applied to all by default.
HTH,
Tom
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From: Collaborative Computational Project in Electron cryo-Microscopy <[log in to unmask]> on behalf of khaja faisal tarique <[log in to unmask]>
Sent: 12 July 2019 05:42:18
To: [log in to unmask]
Subject: [ccpem] Where to find LibG.?
Dear CCPEM users
I am trying to solve the structure of a ribonucleoprotein complex using single particle cryo-EM whose homologous structure is already known. I want to use this to generate restraints for the proteins and RNA molecules and use them in solving the structure of my protein of interest. I am using Prosmart to generate self restrains using CCP-EM suit. It is able to generate restraints for both RNA and protein molecules and I can choose accordingly. Instead of Prosmart I want to use LibG for generating restraints for RNA. However, I am not able to locate LibG in the latest CCP-EM suit. Can anybody please help me in finding LibG and suggest me how it can be linked to the current version of CCP-EM. My other question is: how the restraints generated for RNA in Prosmart different from the one generated by LibG? If it is the same then why do we have separate programs for the same thing?
Best
Faisal Tarique
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