Hi Takanori and other colleagues,
I recently used Relion to do Refine3D job after selecting one good class
from 3D classification. The resolution of this class after 3D
classification was reported at around 3.7 angstrom and the map shows
good density for most side chains of residues. But the quality of map
went worse after I applied this class of particles to Refine3D. I used
these setting:
Symmetry: C4
Number of particles: 81000
Mask during Class3D and Refine3D: none for both jobs(I also tried to
apply one loose mask)
Initial model for Class3D and Refine3D: same initial model from the
output by the last Refine3D job
Initial low pass filtering: 15 angstrom
'solvent flattened FSC' in Refine3D: yes
Could you please provide any suggestions or comments? Thanks a lot!
Best,
KZ
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