Dear James,
10) are the biological questions that you can answer with a (crystal)
structure sufficiently relevant to justify the resources?
Best,
Tim
Am 15.07.2019 21:44, schrieb Holton, James M:
> Hello folks,
>
> I have the distinct honor of chairing the next Gordon Research
> Conference on Diffraction Methods in Structural Biology (July 26-31
> 2020). This meeting will focus on the biggest challenges currently
> faced by structural biologists, and I mean actual real-world
> challenges. As much as possible, these challenges will take the form
> of
> friendly competitions with defined parameters, data, a scoring system,
> and "winners", to be established along with other unpublished results
> only at the meeting, as is tradition at GRCs.
>
> But what are the principle challenges in biological structure
> determination today? I of course have my own ideas, but I feel like
> I'm
> forgetting something. Obvious choices are:
> 1) getting crystals to diffract better
> 2) building models into low-resolution maps (after failing at #1)
> 3) telling if a ligand is really there or not
> 4) the phase problem (dealing with weak signal, twinning and
> pseudotranslation)
> 5) what does "resolution" really mean?
> 6) why are macromolecular R factors so much higher than small-molecule
> ones?
> 7) what is the best way to process serial crystallography data?
> 8) how should one deal with non-isomorphism in multi-crystal methods?
> 9) what is the "structure" of something that won't sit still?
>
> What am I missing? Is industry facing different problems than
> academics? Are there specific challenges facing electron-based
> techniques? If so, could the combined strength of all the world's
> methods developers solve them? I'm interested in hearing the voice of
> this community. On or off-list is fine.
>
> -James Holton
> MAD Scientist
>
>
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--
--
Tim Gruene
Head of the Centre for X-ray Structure Analysis
Faculty of Chemistry
University of Vienna
Phone: +43-1-4277-70202
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