Yes, imagine 1 hr, tabletop, unlimited-length, error-free, dirt-cheap gene synthesis! (With a parallized option for 96-well plates, of course...)
We'll probably just have to wait 10 years.
JPK
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Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
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-----Original Message-----
From: CCP4 bulletin board <[log in to unmask]> On Behalf Of Hargreaves, David
Sent: Wednesday, July 24, 2019 6:01 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] challenges in structural biology
Dear CCP4bb,
From my industrial perspective: The crystallisation bottleneck has probably been fairly well addressed. High throughput screening using robotics and around 20ul of protein per screen is common. There are lots of screens to try even if they are rather redundant in their content. However, I feel there are two things missing: high throughput protein production at microscale and specific tools for construct design. Generally, industry is interested in the druggable site which perhaps is only one domain of a multidomain target protein. Getting a suitably robust crystal system often requires more than one iteration of multiple construct design which in my experience is something of a lottery. Some helpful design tools and an order of magnitude increase in the number of constructs that can be delivered from protein supply would help.
Best wishes,
David
Dr. David Hargreaves
Associate Principal Scientist
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AstraZeneca
Discovery Sciences, Structure & Biophysics Dr David Hargreaves.
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David.Hargreaves @astrazeneca.com
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