Hi Takanori,
It works and is really helpful. Thanks a lot!
Best,
KZ
On 6/19/2019 3:11 PM, Takanori Nakane wrote:
> Hi,
>
> 'Re-center the class averages' must be Yes.
> Otherwise it does not re-create stack files because it suffices
> to refer to the class averages from Class2D.
>
> Best regards,
>
> Takanori Nakane
>
> On 2019/06/19 22:56, KZ wrote:
>> Hi Takanori,
>>
>> I used what you metioned. But there is not mrcs file.
>>
>>
>> Thanks,
>>
>> KZ
>>
>> On 6/19/2019 2:55 PM, Takanori Nakane wrote:
>>> Hi,
>>>
>>> You have to run subset selection with a model STAR file.
>>> Probably you have used a particle STAR file.
>>>
>>> Best regards,
>>>
>>> Takanori Nakane
>>>
>>> On 2019/06/19 22:38, KZ wrote:
>>>> Hi Takanori and other colleagues,
>>>>
>>>> I remember both class_averages.star and class_averages.mrcs
>>>> werewrittenout by Relion after I selected and saved the good
>>>> classes via2Dclassification in the past. Unfortunately,
>>>> class_averages.starshowed upbut there was not class_averages.mrcs
>>>> file when I tried thisusingRelion3 recently. I'd like to try
>>>> template-based particlepicking usingclass_averages.mrcs. Any
>>>> suggestions would be appreciated.
>>>>
>>>>
>>>> Thanks,
>>>>
>>>> KZ
>>>>
>>>>
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