Hi,
'Re-center the class averages' must be Yes.
Otherwise it does not re-create stack files because it suffices
to refer to the class averages from Class2D.
Best regards,
Takanori Nakane
On 2019/06/19 22:56, KZ wrote:
> Hi Takanori,
>
> I used what you metioned. But there is not mrcs file.
>
>
> Thanks,
>
> KZ
>
> On 6/19/2019 2:55 PM, Takanori Nakane wrote:
>> Hi,
>>
>> You have to run subset selection with a model STAR file.
>> Probably you have used a particle STAR file.
>>
>> Best regards,
>>
>> Takanori Nakane
>>
>> On 2019/06/19 22:38, KZ wrote:
>>> Hi Takanori and other colleagues,
>>>
>>> I remember both class_averages.star and class_averages.mrcs were
>>> writtenout by Relion after I selected and saved the good classes via
>>> 2Dclassification in the past. Unfortunately, class_averages.star
>>> showed upbut there was not class_averages.mrcs file when I tried this
>>> usingRelion3 recently. I'd like to try template-based particle
>>> picking usingclass_averages.mrcs. Any suggestions would be appreciated.
>>>
>>>
>>> Thanks,
>>>
>>> KZ
>>>
>>>
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