Dear RELION developer,
I am trying to understand what relion_particle_symmetry_expand is doing. It seems output a star file with rotated symmetry, let's say a C2 symmetry, then the particle number will be doubled due to rotation symmetry applied.Since the mrcs file are remains the same, can I use the star file as an input for refinement? If the case, should I not apply symmetry during the refinement (like go back to C1?). Also, I am particularly interested in helical reconstruction, since in this case the twist and rise seems not taken into account by applying symmetry, while --asu or frac_sampling is used for generating more particles. My first question is, does --asu --frac_sampling do the same thing? My second question is once I use these expanded particles for reconstruction, does it mean I do need to apply helical symmetry? Also, how does helical symmetry implemented during particle expansion?
Best,
Yangqi
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