Hi,
ResMap and RELION use different algorithms to estimate local resolution.
They are not necessarily comparable.
(ResMap tends to be more optimistic in my experience)
> Sorry is there any short anwer for how to make the mask less tight.
Please apply low pass filtering at 15 A and add more soft
edges in MaskCreate.
Best regards,
Takanori Nakane
On 2019/06/13 23:25, Mohamed A Sayed wrote:
> Hi Takanori,
> Thank you for your help. This may answer the FSC curve but even when I use Relion to determine the local resolution on the unfiltered refined 3D model I get 0.4 Ang lower resolution compared to Resmap.
> I used b-factor from post processing and k2 correction curve.
> Sorry is there any short anwer for how to make the mask less tight. Thank you
> ________________________________________
> From: Takanori Nakane <[log in to unmask]>
> Sent: Thursday, June 13, 2019 9:44 PM
> To: Mohamed A Sayed;
> Subject: Re: [ccpem] Resolution estimation
>
> Hi,
>
> Probably your mask is too tight and/or sharp.
>
> Please look at Note 4.16 of
> ftp://ftp.mrc-lmb.cam.ac.uk/pub/scheres/multibody_protocol.pdf.
>
> Best regards,
>
> Takanori Nakane
>
> On 2019/06/13 19:27, Mohamed A Sayed wrote:
>> ​Hello,
>>
>>
>> I hope you would please advise on the following:
>>
>>
>> When I apply post processing in Relion on a refined 3D map, the FSC
>> curve gives 0.3 Angstrom worse resolution than that before post processing.
>>
>>
>> If I use Resmap v 1.95 to estimate the resolution from the
>> unfiltered half maps of the refined model, the resolution is 0.4 A
>> better than when I use the resolution estimation from Relion on the same
>> unfiltered half map.
>>
>>
>> - Which value to believe?
>>
>> - How to calculate FSC without post processing ?
>>
>> - What caused the post processing to give lower resolution and
>> resolution estimation to be lower?
>>
>> Can you please point out for a source on how to reliably fit a pdb into
>> an cryo map?
>>
>> Thank you very much for your help and time.
>>
>>
>>
>>
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>
>
>
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