Hi Takanori,
Thank you for your help. This may answer the FSC curve but even when I use Relion to determine the local resolution on the unfiltered refined 3D model I get 0.4 Ang lower resolution compared to Resmap.
I used b-factor from post processing and k2 correction curve.
Sorry is there any short anwer for how to make the mask less tight. Thank you
________________________________________
From: Takanori Nakane <[log in to unmask]>
Sent: Thursday, June 13, 2019 9:44 PM
To: Mohamed A Sayed;
Subject: Re: [ccpem] Resolution estimation
Hi,
Probably your mask is too tight and/or sharp.
Please look at Note 4.16 of
ftp://ftp.mrc-lmb.cam.ac.uk/pub/scheres/multibody_protocol.pdf.
Best regards,
Takanori Nakane
On 2019/06/13 19:27, Mohamed A Sayed wrote:
> ​Hello,
>
>
> I hope you would please advise on the following:
>
>
> When I apply post processing in Relion on a refined 3D map, the FSC
> curve gives 0.3 Angstrom worse resolution than that before post processing.
>
>
> If I use Resmap v 1.95 to estimate the resolution from the
> unfiltered half maps of the refined model, the resolution is 0.4 A
> better than when I use the resolution estimation from Relion on the same
> unfiltered half map.
>
>
> - Which value to believe?
>
> - How to calculate FSC without post processing ?
>
> - What caused the post processing to give lower resolution and
> resolution estimation to be lower?
>
> Can you please point out for a source on how to reliably fit a pdb into
> an cryo map?
>
> Thank you very much for your help and time.
>
>
>
>
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