Hi Jing,
You also need to use the --new_box flag, to get the rescaled model in the right-sized box.
Regards,
Daniel
> On 24 Jun 2019, at 18:55, Jing Liu <[log in to unmask]> wrote:
>
> Hi all,
>
> I have some problem in rescaling an initial model for 3D classification. I have a medium resolution structure from a data set that the box size is 256 and pixl is 1.49 (I extract the particle with box 360 and downscale to 256, the original pixl is 1.06). Then I tried to autopick more particles in the same data set to try to increase the resolution. And the particles I picked are now extracted at 256 but down scaled to 128 so the pixl size is 2.12. I try to use the medium resolution structure as an initial model to do 3D classification. So I use relion image handler to do the following: relion_image_handler --i run_class001.mrc --angpix 1.49 \ --rescale_angpix 2.12 --o ./run_class001_2p02.mrc
>
> I am not sure what I did wrong. But the 3D classification message said that the image size is not the same. Can anyone give me some idea?
>
> Thank you very much in advance.
>
> Best.
>
> Jing
>
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