Hi all,
I have some problem in rescaling an initial model for 3D classification. I have a medium resolution structure from a data set that the box size is 256 and pixl is 1.49 (I extract the particle with box 360 and downscale to 256, the original pixl is 1.06). Then I tried to autopick more particles in the same data set to try to increase the resolution. And the particles I picked are now extracted at 256 but down scaled to 128 so the pixl size is 2.12. I try to use the medium resolution structure as an initial model to do 3D classification. So I use relion image handler to do the following: relion_image_handler --i run_class001.mrc --angpix 1.49 \ --rescale_angpix 2.12 --o ./run_class001_2p02.mrc
I am not sure what I did wrong. But the 3D classification message said that the image size is not the same. Can anyone give me some idea?
Thank you very much in advance.
Best.
Jing
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