Hi,
You have to run subset selection with a model STAR file.
Probably you have used a particle STAR file.
Best regards,
Takanori Nakane
On 2019/06/19 22:38, KZ wrote:
> Hi Takanori and other colleagues,
>
> I remember both class_averages.star and class_averages.mrcs were written
> out by Relion after I selected and saved the good classes via 2D
> classification in the past. Unfortunately, class_averages.star showed up
> but there was not class_averages.mrcs file when I tried this using
> Relion3 recently. I'd like to try template-based particle picking using
> class_averages.mrcs. Any suggestions would be appreciated.
>
>
> Thanks,
>
> KZ
>
>
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