Hi,
> Is there a quantitative way to estimate their values?
Look at the rlnBeamTiltX and Y columns in the particle STAR file.
The unit is mrad.
> how to fix this during data acquisition from the microscope?
Please study "coma free alignment".
Best regards,
Takanori Nakane
On 2019/06/13 21:17, Mohamed A Sayed wrote:
>
> Hello
>
> Please look at the attached beam tilt delta phase diagrams. The linear
> fit diagram shows bright and dark corners on one diagonal. Is there
> a quantitative way to estimate their values? Is this severe beam tilt
> and how to fix this during data acquisition from the microscope? Thank
> you very much for your help.
>
>
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