Dear colleagues,
Please note we've made a minor update release to RELION-3.0 (now 3.0.6).
Changes are below. The changes to the LOG-picker are probably of most
direct influence, as this works better now, but the optimal LoG
threshold may have changed for your data.
Happy processing!
Sjors & Takanori
Changes in 3.0.6:
- RelionCor supports dose weighting of datasets at 100 kV.
- The inter-particle distance cutoff in the Laplacian-of-Gaussian (LoG)
picker was improved.
When LoG picker is enabled, `minimum inter-particle distance` in the
"autopicking" tab is not used. This is by design. Instead, neighbouring
particles within
(detected diameter at the point + `--LoG_diam_min`) *
`--LoG_neighbour` / 100
are avoided. The default for `--LoG_neighbour` is 100 %. You can make
this smaller or larger to pick more or less, respectively.
- Implemented `--LoG_upper_threshold` option.
LoG picker tended to pick high contrast contamination like ice and
ethane droplets. They have higher scores than real particles, so `adjust
default threshold` is useless. Now we have `--LoG_upper_threshold`
option to set an upper limit in scores. Useful values are typically 1.5
to 4. The default is 99999 (i.e. no limit in practice)
This release also contains the following bug fixes.
- PostProcess: fixed a bug where `postprocess_masked.mrc` is overwritten
by `half2_masked.mrc` when `--half_maps` is provided (reported by
@keitaroyam).
- Stability of histogram generation was improved.
- Prevented "too long arguments" errors in scratch (issue #467 reported
by @EMBL-Structures).
- `relion_star_handler --split`: removed an assumption on the number of
digits.
- AutoPicker: rejects 3D references for filaments
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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