Apologies, my trivial mistake. I had forgotten to set ‘coordinates are start-end only’ to yes in the Helix tab.
Luca
> On 23 May 2019, at 12:14, Luca Pellegrini <[log in to unmask]> wrote:
>
> Hello,
>
> I am running particle extraction of helical segments after manual picking. Relion 3.0 gives the following error, complaining that prior information of helical segments is missing:
>
> ==================
> ERROR:
> helix.cpp::convertHelicalSegmentCoordsToMetaDataTable(): Prior information of helical segments is missing in ManualPick/job008/Movies/Images-Disc1/GridSquare_25124405/Data/FoilHole_25125958_Data_25126738_25126739_20180325_1400_Fractions_manualpick.star
> --------------------------------------------------------------------------
> MPI_ABORT was invoked on rank 0 in communicator MPI_COMM_WORLD
> with errorcode 1.
>
> NOTE: invoking MPI_ABORT causes Open MPI to kill all MPI processes.
> You may or may not see output from other processes, depending on
> exactly when Open MPI kills them.
> --------------------------------------------------------------------------
> (END)
>
>
> Can anyone please advise how to fix this? I can’t see anything obviously wrong with the *_manualpick.star files.
>
> Relion also issues a warning that the *_manual_pick.star files for micrographs that I haven’t manually picked don’t exist…
>
> Apologies if there’s a trivial explanation that I have missed.
>
> Thank you and best regards,
> Luca
>
>
>
>
> Luca Pellegrini, PhD
> Department of Biochemistry
> University of Cambridge
> Cambridge CB2 1GA
> United Kingdom
>
> [log in to unmask]
> tel.: 0044 1223 760469
> https://www.bioc.cam.ac.uk/pellegrini-group/pellegrini-group
> @PellegriniLab
>
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Luca Pellegrini, PhD
Department of Biochemistry
University of Cambridge
Cambridge CB2 1GA
United Kingdom
[log in to unmask]
tel.: 0044 1223 760469
https://www.bioc.cam.ac.uk/pellegrini-group/pellegrini-group
@PellegriniLab
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