Hi,
Please look at
https://www3.mrc-lmb.cam.ac.uk/relion/index.php?title=Conventions_%26_File_formats#Coordinate_system.
Angles are usually from -180 to +180 deg.
I think values beyond this range are accepted
but I am not sure (please test yourself).
With symmetry, angular search is limited to the asymmetric unit
on the unit sphere. Relevant codes are here:
https://github.com/3dem/relion/blob/master/src/healpix_sampling.cpp#L2001
Best regards,
Takanori Nakane
On 2019/05/30 19:25, Yanyan Zhao wrote:
> Hi, Sorry that mislabeled two files in the previous message. I resend it
> again. I'm wondering how Euler angle in RELION is defined. To be
> specific, I have these questions. What is Euler angle (0,0,0)? How does
> euler angle change in terms of 360 degree? For example, for Rot angle,
> if I rotate around Z axis clockwise, does the rot keep increasing from 0
> to 180? and what happens beyond 180 degree? If I have a reconstruction
> with D8 symmetry. Now I like to know how euler angle changes when I
> focus on two subunits with D1 symmetry in this reconstruction. I
> attached a .star file for euler angle information of one particle after
> D8 symmetry release.
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