Hi,
Please look at Max's excellent post at
https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=CCPEM;fd0e7fab.1810.
Best regards,
Takanori Nakane
On 2019/04/01 20:05, Laurence Pearl wrote:
> After loads of careful 2D and 3D re-classification, we’ve managed to get
> a subset of ~30k particles from a Krios data collection, that refine in
> RELION3 to a 3D structure at 5-6Å. After map sharpening we can locate
> and fit lots of secondary structural elements, but obviously no sequence
> can be located and the polarity of the secondary structure elements
> can’t be defined with certainty.
>
> To extend the resolution to the point we might be able to fit some
> sequence, we have collected a further ~2000 images from the same sample
> with the intention of merging particles from the old and new data. The
> new data was collected on a different Krios with parameters as close to
> the first set as possible - but there will be a tiny difference in pixel
> size.
>
> Can anyone suggest optimal workflows for extracting the right subset of
> particles from the new images and effectively combining them with the
> previous particles to achieve a higher resolution ?
>
> Many possibilities and combinations spring to mind, but as I’m old I’m
> keen not to have to rediscover the wheel on this, so would be very
> grateful for suggestions born of good experience.
>
> Laurence
> ----------------------------------------------------
> Laurence H. Pearl PhD FRS FMedSci
>
> Professor of Structural Biology
> Genome Damage and Stability Centre
> University of Sussex, Brighton, BN1 9RQ, UK
>
> Phone +44-(0)1273 678 349
> ----------------------------------------------------
> "Live Modestly and do Serious Things .. "
> - Dorothy Crowfoot Hodgkin
> ----------------------------------------------------
>
>
>
>
>
> ------------------------------------------------------------------------
>
> To unsubscribe from the CCPEM list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
>
########################################################################
To unsubscribe from the CCPEM list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
|