Hi,
At 14 A, there is no point running Polishing (yet) and
you cannot reliably fit B factors.
If you believe the resolution is limited by the defocus gradient,
run several cycles of CtfRefine and Refine3D (see hemagglutinin example
in our RELION3 paper) or use tilt-aware CTF estimation programs
(e.g. Warp, CTFTILT, GoCTF).
Best regards,
Takanori Nakane
On 2019/03/26 21:30, Zhao, Haiyan wrote:
> Because it is a tilt 40 degree data set on K3 detector with 200KV Arctic, so far the resolution is 14A after 3D refinement
> Resolution range is default,
> Dose rate is 1.34 e-/A2,
>
>
> Haiyan Zhao
> Department of Biochemistry and Molecular Genetics
> Voice: 858-869-3633
>
>
>
>
>
>
>
> On 3/26/19, 3:17 PM, "Takanori Nakane" <[log in to unmask]> wrote:
>
>> Hi,
>>
>> What is the resolution of your structure?
>> What is the resolution range you used to fit B factors?
>> What is the dose rate?
>>
>> Best regards,
>>
>> Takanori Nakane
>>
>> On 2019/03/26 20:41, Zhao, Haiyan wrote:
>>> I collected a tilt data set, when I run bayesian polishing for all
>>> particles, I got the b factor like I attached the pdf file, I don’t know
>>> what happened? Any suggestions. Thanks
>>>
>>> Haiyan Zhao
>>>
>>> Department of Biochemistry and Molecular Genetics
>>>
>>> Voice: 858-869-3633
>>>
>>>
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