Resolution range is
+ maximum frequency to consider for alignment: 20.72 A (30 px)
+ frequency range to consider for evaluation: 20.72 - 14.8 A (30 - 42 px)
Best wishes,
Haiyan Zhao
Department of Biochemistry and Molecular Genetics
Voice: 858-869-3633
On 3/26/19, 3:30 PM, "Collaborative Computational Project in Electron cryo-Microscopy on behalf of Zhao, Haiyan" <[log in to unmask] on behalf of [log in to unmask]> wrote:
>Because it is a tilt 40 degree data set on K3 detector with 200KV Arctic, so far the resolution is 14A after 3D refinement
>Resolution range is default,
>Dose rate is 1.34 e-/A2,
>
>
>Haiyan Zhao
>Department of Biochemistry and Molecular Genetics
>Voice: 858-869-3633
>
>
>
>
>
>
>
>On 3/26/19, 3:17 PM, "Takanori Nakane" <[log in to unmask]> wrote:
>
>>Hi,
>>
>>What is the resolution of your structure?
>>What is the resolution range you used to fit B factors?
>>What is the dose rate?
>>
>>Best regards,
>>
>>Takanori Nakane
>>
>>On 2019/03/26 20:41, Zhao, Haiyan wrote:
>>> I collected a tilt data set, when I run bayesian polishing for all
>>> particles, I got the b factor like I attached the pdf file, I don’t know
>>> what happened? Any suggestions. Thanks
>>>
>>> Haiyan Zhao
>>>
>>> Department of Biochemistry and Molecular Genetics
>>>
>>> Voice: 858-869-3633
>>>
>>>
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