Dear Pranav,
we never used the estimated gain for correcting the movies only for
monitoring, but it will probably be a good substitute. In scipion you
can estimate the gain for each movie (or for one movie and assume it is
the same for all of them) using the algorithm described in
https://www.ncbi.nlm.nih.gov/pubmed/29551714
Kind regards, Carlos Oscar
El 14/03/2019 a las 6:24, Pranav Shah escribió:
> Dear Community,
>
> Due to an inadvertent mistake I ended up collecting a dataset
> normalised with a screwey gain ref and I was wondering if there was an
> in silico way to fix this mistake. Has anyone had a similar problem
> and managed to save their precious data?
>
> Looking forward to your suggestions.
> Cheers,
> Pranav
> --
> Pranav Shah
> Postdoctoral Research Fellow.
>
> Hogle Lab
> Department of Biological Chemistry and Molecular Pharmacology,
> Building C2 - First Floor
> 240 Longwood Avenue
> Boston, MA 02115
> (617) 432-4360 (fax)
> (617) 432-3839 (lab)
>
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--
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Carlos Oscar Sánchez Sorzano e-mail: [log in to unmask]
Biocomputing unit http://i2pc.es/coss
National Center of Biotechnology (CSIC)
c/Darwin, 3
Campus Universidad Autónoma (Cantoblanco) Tlf: 34-91-585 4510
28049 MADRID (SPAIN) Fax: 34-91-585 4506
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