Le Jeudi 28 Mars 2019 12:03 CET, jai mohan <[log in to unmask]> a écrit:
A friend of mine (Angel Piñeiro) just suggested to me the "Maragoni effect", which is likely active in the dancing droplets from Stanford and might explain the "explosion" into separate bubbles....
Indeed, one can see on Wikipedia a video illustrating how the contact of fluids with different surface tensions can have big effects...
> May be, I do co-relate your crystal pic with Manu Prakash at Stanford on his work on Dancing Droplets, briefing the surface tension and evaporation ^ the rule of two component fluids. # Since your precipitant contain PVP a shape controlling agent #https://med.stanford.edu/news/all-news/2015/03/researchers-solve-mystery-of-the-dancing-droplets.html
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> Best wishes
> S.M.Jaimohan PhD
> On Thursday, 28 March, 2019, 1:54:23 pm IST, Sergei Strelkov <[log in to unmask]> wrote:
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> #yiv3861306982 #yiv3861306982 --P{margin-top:0;margin-bottom:0;}#yiv3861306982
> Artem (and Beatriz),
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> Me bad, could have thought about that! I think you are right.
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> There were initially bubbles in each drop (7 in one case, 4 in the other).
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> At some point the bubbles exploded (it was an instantaneous process, not just shrinking).
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> Kind regards,
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> Sergei
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> Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of Pharmaceutical Sciences, KU Leuven O&N2, Campus Gasthuisberg, Herestraat 49 bus 822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 32 Lab pages: http://pharm.kuleuven.be/BiocrystallographyFrom: CCP4 bulletin board <[log in to unmask]> on behalf of Artem Evdokimov <[log in to unmask]>
> Sent: Thursday, March 28, 2019 1:07
> To: [log in to unmask]
> Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat!
> Looks like multiple adjacent bubbles that were initially touching but eventually shrunk down to the central cores - the connectors are protein filaments (skin on the bubbles) left over from when bubbles had contact points.
> Artem
> On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville) <[log in to unmask]> wrote:
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> Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition is found in both Index and JCSG screens as well as Classics II.
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> Bevan Marshall
> Staff Scientist | Collaborative Crystallisation Centre
> Manufacturing
> CSIRO
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> From: CCP4 bulletin board [mailto:[log in to unmask]]On Behalf Of LEGRAND Pierre
> Sent: Thursday, 28 March 2019 9:13 AM
> To: [log in to unmask]
> Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
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> Dear Beatriz,
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> Nice drops :-))
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> Could it be that there is a reaction going on in these drops ?
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> The conditions are quite "exotic" with possibilities of coordination or oxydoreduction (Co2+/Co3+) or polymerization...
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> Do you have reductants with the protein buffer ?
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> Is the protein an enzyme or a metalloprotein ?
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> Just some ideas.
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> Best wishes,
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> Pierre
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> De : CCP4 bulletin board [[log in to unmask]] de la part de Beatriz Gomes Guimaraes [[log in to unmask]]
> Envoyé : mercredi 27 mars 2019 19:44
> À : [log in to unmask]
> Objet : [ccp4bb] Interesting pattern on a crystallization drop
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> Dear all,
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> I would like to share with you a surprising pattern I found when examining some crystallization plates (attached figures).
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> It is less obvious looking the photos, but apparently the "lines" are formed by precipitated protein and there are some "bubbles" with small drops inside.I wish they were microcrystals but I do not think this is the case.
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> I was suprised by the symmetry !
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> And it is not completely random because for the same condition the difference between the two drops are : protein alone ("hexagon") and protein + ligand ("rhombus")
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> crystallization condition is:
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> 0.01 M Cobalt(II) chloride hexahydrate
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> 0.1 M Tris pH 8.5
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> 20% w/v Polyvinylpyrrolidone K 15
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> Have you seen anything similar before?
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> Thank you for your comments!
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> Beatriz
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> --------------------------
> Beatriz Guimarães
> Laboratory of Structural Biology and Protein Engineering
> Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
> Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
> CIC 81350-010
> Curitiba - PR, Brasil
> Tel.:+55(41)3316-3225/2104-3438
>
>
>
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