I have no answer for this case, but I have a similar situation in which the 3D classification separates my particles based on their orientation. It is quite evident when looking at the angdist.bild files in chimera.
Does anybody have a similar situation? And a way to fix this behavior?
Herve
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Herve Celia [C]
NIDDK NIH
Laboratory of Molecular Biology
50 South Drive, Room 4511
Bethesda, MD 20892-8030
Phone (+1) 301-451-6656
Fax (+1) 301-480-0597
Cell (+1) 301-204-0300
On 2/27/19, 5:38 AM, "Joe Atherton" <[log in to unmask]> wrote:
Hi,
I have an interesting situation where after performing 3D classification on 3 data sets, one on a graphene oxide substrate, I have class separation based on the substrate (all graphene oxide particles go into one class, the others go into the others). I would be ok with this if I was convinced that the graphene oxide dataset represents a different conformation or structure, but this appears not to be the case. I should note that the graphene oxide and in ice data sets give different orientation preferences.
Does anyone have any insight or any suggestions to get around this problem?
Cheers,
Joe
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