Dear Samuel,
I'm guessing you are not constrained by sample availability. Consider a multi-crystal approach to understand your native structure and contrast against structures with low occupancy binders (Pearce et al 2017, https://www.nature.com/articles/ncomms15123 ).
That structure (a pandda ground state model) will be a great place to start a polder map.
Jose'
===
Jose Brandao-Neto MPhil CPhys
Senior Beamline Scientist - I04-1
XChem Scientist
Diamond Light Source
[log in to unmask]
+44 (0)1235 778506
===
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
|