Hi,
Please show me the FSC table in the first part of your run_model.star
from Refine3D.
Best regards,
Takanori Nakane
On 2019/02/03 14:57, sunyeping wrote:
> Dear All,
> I post this problem yesterday but haven't got any anwer. I think I should give more detail.
>
> In the Refine3D, I get the warning message as following:
>
> WARNING: FSC dipped below 0.5 and rose again. Using higher resolution of 8.37931 A, instead of 9 A.
> This is not necessarily a bad thing. Often it is caused by too tight masks.
> WARNING: FSC dipped below 0.5 and rose again. Using higher resolution of 8.67857 A, instead of 9.34615 A.
> This is not necessarily a bad thing. Often it is caused by too tight masks.
> WARNING: FSC dipped below 0.5 and rose again. Using higher resolution of 5.65116 A, instead of 6.1519 A.
> This is not necessarily a bad thing. Often it is caused by too tight masks.
> WARNING: FSC dipped below 0.5 and rose again. Using higher resolution of 5.65116 A, instead of 5.92683 A.
> This is not necessarily a bad thing. Often it is caused by too tight masks.
> I have tried to increase the mask
>
> In PostProcess job, I got the following error message in the run.err file:
>
> in: /home/cryoem/softwares/Relion30Beta/relion/src/postprocessing.cpp, line 1152
> === Backtrace ===
> /home/cryoem/softwares/Relion30Beta/relion/build/bin/relion_postprocess(_ZN11RelionErrorC1ERKSsS1_l+0x41) [0x423f01]
> /home/cryoem/softwares/Relion30Beta/relion/build/bin/relion_postprocess(_ZN14Postprocessing3runEv+0xbaa) [0x44896a]
> /home/cryoem/softwares/Relion30Beta/relion/build/bin/relion_postprocess(main+0x62) [0x419a02]
> /lib64/libc.so.6(__libc_start_main+0xf5) [0x7f729de9db35]
> /home/cryoem/softwares/Relion30Beta/relion/build/bin/relion_postprocess() [0x419f6f]
> ==================
> ERROR:
> Postprocessing::run ERROR: FSC curve never drops below randomize_fsc_at.
>
> And the message int the run.out file of the PostProcess job is:
>
>
> == Reading input half-reconstructions:
> + half1-map: Refine3D/job068/run_half1_class001_unfil.mrc
> + half2-map: Refine3D/job068/run_half2_class001_unfil.mrc
> == Using a user-provided mask ...
> + input mask: MaskCreate/job077/mask.mrc
> == Masking input maps ...
> + randomize phases beyond: -486 Angstroms
>
> Do you know how is this " "Postprocessing::run ERROR: FSC curve never drops below randomize_fsc_at." problem" caused and how to solve it?
> I will be grateful if you can help me with this.
>
> Best Regards.
> Yeping
>
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