Hi,
Did you make sure there are no duplicated particles?
Best regards,
Takanori Nakane
> Hi Ashu,
>
> Are you imposing symmetry?
>
> P
>
>> Il giorno 4 gen 2019, alle ore 18:31, Ashu Sharma
>> <[log in to unmask]> ha scritto:
>>
>> Dear All,
>>
>> On a single particle cryo-EM data refined in relion 2, I wanted to do
>> Bayesian polishing. And therefore after MotionCorr2 etc. in Relion 3
>> pipe line, I did a 3D refinement on exactly the same set of particles
>> which I used in Relion 2 for auto-refine.
>> Refinement converges to the same resolution as Relion 2, map looks the
>> same. But in Relion 3 post-processing, none of the FSC curves except the
>> one for phase-randomized masked maps ever touches zero. They plateau
>> around 0.02-0.03. I tried playing with wider/softer masks but that
>> doesn't help.
>>
>> What could be going wrong?
>>
>> Thanks a lot !
>> Ashu
>>
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