Hi Takanori,
thanks for clarifying!
bw Dieter
------------------------------------------------------------------------
Dieter Blaas,
Max F. Perutz Laboratories
Medical University of Vienna,
Inst. Med. Biochem., Vienna Biocenter (VBC),
Dr. Bohr Gasse 9/3,
A-1030 Vienna, Austria,
Tel: 0043 1 4277 61630,
Fax: 0043 1 4277 9616,
e-mail: [log in to unmask]
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Am 23.01.2019 um 17:53 schrieb Takanori Nakane:
> Hi,
>
> Cropping is not implemented.
> Cropping is no longer necessary for newer versions of Gctf.
>
> If you need to re-extract "older refined coordinates" from cropped
> micrographs, please use UCSF MotionCor2 with -Crop.
>
> Best regards,
>
> Takanori Nakane
>
>> Dear all,
>>
>> ??? in Shawn Zheng's MotionCor2 it was necessary to crop micrographs
>> from K2 with the parameter "-crop 3660 3660" during the run to be
>> correctly process by Gctf called from Relion-2.
>>
>> Micrographs preprocessed with the Relion-3 implementation of motioncorr
>> are not cropped (i.e. "xmipp_image_header" issues: "Dimensions???? : 1 x
>> 1 x 3838 x 3710"), which apparently gives rise to problems when trying
>> to re-extract older refined coordinates.
>>
>> Is there a way of cropping the micrographs in Relion-3 running
>> motioncorr? "-crop 3660? 3660" or "--crop 3660 3660" is not accepted by
>> "Additional Arguments".
>>
>> Thank you for help,
>>
>> bw Dieter
>>
>> ------------------------------------------------------------------------
>> Dieter Blaas,
>> Max F. Perutz Laboratories
>> Medical University of Vienna,
>> Inst. Med. Biochem., Vienna Biocenter (VBC),
>> Dr. Bohr Gasse 9/3,
>> A-1030 Vienna, Austria,
>> Tel: 0043 1 4277 61630,
>> Fax: 0043 1 4277 9616,
>> e-mail: [log in to unmask]
>> ------------------------------------------------------------------------
>>
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