We used to use dichlorodimethylsilane in toluene to siliconize both
coverslips and the special glass capillaries for crystal mounting.
I don't have the protocol we used anymore, but the one listed on
protocolpedia sounds familiar.
https://www.protocolpedia.com/blog/2017/05/11/siliconized-coverslips-2/
I recall we often baked the items afterwards.
We used to use staining racks to hold the coverslips and a staining
vessel to hold the solution (in a hood). The racks looked like
https://www.thomassci.com/Equipment/Histology/_/THOMAS-COVER-GLASS-STAINING-OUTFITS?q=Cover%20Glass%20Rack
it looks like sigma also has a poly-propylene rack that is much
less expensive,
https://www.sigmaaldrich.com/catalog/product/sigma/z688568?lang=en®ion=US&cm_sp=Insite-_-recent_fixed-_-recent5-5
but they may be incompatible with toluene.
Regards,
Mitch
Quoting Zhijie Li <[log in to unmask]>:
> Hi,
>
> I believe that one can put a 50-100uL drop of fresh SigmaCote (in a
> tube cap) with the glass pieces (surface well exposed), sealed in a
> dedicated (because the container will be coated too) container
> (air-tight lunch boxes). After a while the SigmaCote vapor should
> react with the glass and bring polysiloxane groups to the glass
> surface. I have done this with microscope slips. To make a few
> hundred .22 mm cover slips I think the major challenge is to make a
> frame for supporting the separated (~1mm apart to allow free
> diffusion?) cover slips (3D print?). Dry paper may work too. If some
> of the cover slips tend to stick together, a little vacuuming can
> help.
>
> SigmaCote is a chlorinated polysiloxane. The Cl-Si group allows it
> to react with the hydroxyls on glass surface. Just be aware that the
> same group reacts even more readily with water in air, making the
> reagent less reactive with glass overtime (but I have used a VERY
> old bottle and it worked).
>
> References:
>
> https://en.wikipedia.org/wiki/Chlorosilane
>
> https://pubs.acs.org/doi/pdfplus/10.1021/j100727a046
>
> https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/sl2pis.pdf
>
> Zhijie
>
>
> On 31/01/2019 4:16 a.m.,
> [log in to unmask]<mailto:[log in to unmask]> wrote:
> A long time ago, before siliconized coverslips became commercially
> available, we used to siliconize coverslips ourselves. It is not
> really that much work and unsiliconized cover slips should be very
> cheap. If you wish, I could try to find back the protocol.
> Best,
> Herman
>
> Von: CCP4 bulletin board [mailto:[log in to unmask]] Im Auftrag
> von Rajnandani Kashyap
> Gesendet: Donnerstag, 31. Januar 2019 09:17
> An: [log in to unmask]<mailto:[log in to unmask]>
> Betreff: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized
> glass coverslips for crystallization?
>
> Dear All
> I am a PhD student who requires lots of coverslips (!!) for setting
> up hanging drop crystallization. The company sells it for a huge
> amount. Also there is a wide monetary difference between a normal
> siliconized coverslip and a 22mm siliconized circle coverslips. We
> tried to search for an alternative companies but couldn't get any
> one who sells coverslips with the same dimensions (0.19-0.22mm glass
> thickness and 22 mm glass diameter). Is there any alternative
> company (distribution in India) from where we can buy them for a
> reasonable price?
> Thanks in advance for sparing your valuable time and efforts.
>
> Regards
> Rajnandani Kashyap
> India
>
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