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CCPEM  December 2018

CCPEM December 2018

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Subject:

Re: Deep Consensus and Scipion/Xmipp

From:

Dieter Blaas <[log in to unmask]>

Reply-To:

Dieter Blaas <[log in to unmask]>

Date:

Mon, 17 Dec 2018 00:11:59 +0100

Content-Type:

text/plain

Parts/Attachments:

Parts/Attachments

text/plain (138 lines)

Dear Ruben and Carlos Oscar,

    thank you very much! I'll try and report but I am afraid that it 
will take some time.....

bw Dieter

------------------------------------------------------------------------
Dieter Blaas,
Max F. Perutz Laboratories
Medical University of Vienna,
Inst. Med. Biochem., Vienna Biocenter (VBC),
Dr. Bohr Gasse 9/3,
A-1030 Vienna, Austria,
Tel: 0043 1 4277 61630,
Fax: 0043 1 4277 9616,
e-mail: [log in to unmask]
------------------------------------------------------------------------

Am 16.12.2018 um 22:15 schrieb Carlos Oscar S. Sorzano:
> Dear Dieter,
>
> DeepConsensus has been integrated into Scipion but it has not been 
> included in the scipion current release yet. At some moment in the 
> near future, the release of the packages will be different from the 
> release of Scipion. The different packages will be plugins and you 
> will be notified when new plugins or newer versions are available.
>
> At the moment, in order to use it you, have to switch to co_tensor_new 
> development branch. I am afraid that it cannot be executed in command 
> line.
> If you want to install scipion version with DeepConsensus integrated, 
> you just need to use the following commands ( It will be almost 
> automatic once the release will include DeepConsensus).
>
>
> WARNING, in order to use GPU acceleration you will need CUDA-8.0 and 
> CUDNN-6
> or CUDA-9.0 and CUDNN-7  (https://developer.nvidia.com/cudnn).
> Basically, to install CUDNN you just have to uncompress and copy to 
> CUDA path subfolds lib64 and lib the contained fields
>
> #Install scipion and xmipp to try deepConsensus before release
>
> Suppose we want to install scipion in /home/ruben/app/scipion and 
> xmipp in /home/ruben/app/xmipp_bundle
>
> #First install xmipp
>
> cd /home/ruben/app/
> mkdir xmipp_bundle
> cd xmipp_bundle
>
> wget https://raw.githubusercontent.com/I2PC/xmipp/devel/xmipp -O xmipp
> chmod a+x xmipp
> ./xmipp all N=8 br=co_tensor_new
> #Edit xmipp.conf. to use CUDA e.g. with gedit or nano
> nano xmipp.conf
>   CUDA=True
>
> #Then, install scipion
> cd /home/ruben/app/
> git clone https://github.com/I2PC/scipion
> cd scipion
> git checkout co_tensor_new_new
> ./scipion config
> #Edit ./config/scipion.conf. to use CUDA e.g. with gedit or nano
> nano config.scipion.conf
>   CUDA_LIB = /home/ruben/app/cudas/cuda-8-CDNN_6/lib64
>   CUDA_BIN = /home/ruben/app/cudas/cuda-8-CDNN_6/bin
>   NVCC_INCLUDE = /home/ruben/app/cudas/cuda-8-CDNN_6/include
>   CUDA=True
>
> #Tell scipion where xmipp is
> ./scipion installp -p 
> /home/ruben/app/xmipp_bundle/src/scipion-em-xmipp --devel --noBin
> ln -s /home/ruben/app/xmipp_bundle/src/scipion-em-xmipp/xmipp3 
> /home/ruben/app/scipion/software/lib/python2.7/site-packages/
> #Edit ~/.config/scipion/scipion.conf to tell where xmipp binaries are
> nano -w ~/.config/scipion/scipion.conf
>   XMIPP_HOME = /home/ruben/app/xmipp_bundle/build
>
> #Finally, install deepConsensus
> ./scipion installb deepLearnigToolkit
>
>
> Now you can create scipion project by
>
> ./scipion
>
> The GUI will be launched
>
> In order to use deepConsensus, import the micrographs, the particles, 
> possibly pick with another picker
> and fill the protocol form for deepConsensus.
> To find it easily you can use ctrl+f
> and type DeepConsensus
>
> If you have any further problem, please do not hesitate to tell us.
>
> Kind regards, Ruben and Carlos Oscar
>
> El 16/12/2018 a las 8:26, Dieter Blaas escribió:
>> Dear Carlos and all,
>>
>>     I was wondering how to use DeepConsensus for the elimination of 
>> already picked 'bad' virus particles instead of cleaning the data set 
>> (in relion) with multiple Class3D runs and cutting off 
>> _rlnLogLikeliContribution outlayers. Since the paper on Deep 
>> Consensus states that the program had been integrated into Scipion I 
>> upgraded my installation following the description 
>> (https://github.com/I2PC/scipion/wiki/How-to-Install). However, it 
>> still reports "version-1.1.beta". Even after a completely new install 
>> the version did not change. Can anybody please indicate whether it is 
>> possible to run DeepConsensus on a relion star file and if so how? 
>> I'd very much like to use it in the old-fashioned way from command 
>> line :-).
>>
>> Thanks,  bw Dieter
>>
>> ------------------------------------------------------------------------
>> Dieter Blaas,
>> Max F. Perutz Laboratories
>> Medical University of Vienna
>> Inst. Med. Biochem., Vienna Biocenter (VBC),
>> Dr. Bohr Gasse 9/3,
>> A-1030 Vienna, Austria,
>> Tel: 0043 1 4277 61630,
>> Fax: 0043 1 4277 9616,
>> e-mail: [log in to unmask]
>> ------------------------------------------------------------------------
>>

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