Hi Joe,
you can also reduce map size with ARP/wARP (version 8.0, available with
latest CCP4 update). We didn't expect that it might be useful for
anyone, but the program uses reduced-size maps internally. You can get
them e.g by running a simple map pre-processing job forcing input map
B-factor (--badd 0) and keeping intermediate files (--keepjunk):
auto_em.sh --process --keepjunk --resmax [resolution] --mapin [map
file] --prot_residues [number of protein residues] --badd 0
it will regenerate a [map file]_shifted.map file in a project directory
covering only expected extent of the molecule (for an input number of
residues). Alternatively, if you have a model already, you can run a
refinement job with --keepjunk option. Then, both map and input model
will be placed in a smaller box.
hope it helps,
grzegorz
On 10/12/18 23:46, Joseph Batchelor wrote:
> Dear CCPEM community,
>
> Can someone kindly point us towards a method to reduce the size of a cryo-EM map to facilitate sharing results?
>
> Thank you,
> Joe
>
> ########################################################################
>
> To unsubscribe from the CCPEM list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
########################################################################
To unsubscribe from the CCPEM list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
|