Use CCP-EM! Which has your favourite CCP4 programs adapted for cryoEM, plus some others. See www.ccpem.ac.uk<http://www.ccpem.ac.uk>
There is a hierarchy from rigid body fitting (of your xtal monomer), to flexible fitting (to account for adjustments in the new form), to de novo building and refinement. What you can do will depend on the EM resolution (local as well as global).
Look out for our annual Icknield workshops that provide training on model fitting and building.
HTH
Martyn
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Monika Pathak
Sent: 04 December 2018 11:04
To: ccp4bb
Subject: [ccp4bb] fit pdb on cryo-EM map
Hi there
Please can I get some suggestion on how to build a pdb into a cryoEM map.
To be more clear I have a crystal structure solved as dimer in asymmetric unit and I am trying to model it as a trimer form (biologically relevant) using cryo-EM map of a very similar homologue protein as a template.
I thank in advance for your help.
Thanks
Regards
Monika
School of Life Sciences
University of Nottingham
UK
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