Hi,
I am afraid that you probably have to manually tweak STAR files.
The easiest way is to re-extract particles from newly motion-corrected
images and re-refine.
Best regards,
Takanori Nakane
On 2018/11/18 10:53, Yehuda Halfon wrote:
> Hi all,
>
> We have some data that we wanted to use the new tools from relion 3 on it.
> In order to save time we did motioncorr again using the relion implementation, then we used the old data from relion 2.1 to do the 3D refine and Post processing for the bayesian polishing and CTF refinement.
>
> Then we did the CTF refinement which went well. But when we tried to use bayesian polishing (training) using the micrographs from the relion 3 implementation motioncorr; Particles CtfRefine and Postprocess Star file (that we did in relion 3). We get the following error:
>
> ERROR: MicrographHandler::getMetaName: no metadata star-file for
> (*here is the path for the mrc which is deleted for secrecy*) found in MotionCorr/job004/corrected_micrographs.star.
>
> What am I doing wrong?
> Can't I skip the steps and will need to do it all again?
>
> Yehuda
>
> P.s
> When I used relion 3 from the beginning to the end before everything worked fine.
>
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