Hi,
> Thanks a lot. Just to be clear, for Bayesian polishing, I use the
combined
> postprocessed star file for each data set separately?
Yes. You should use the same post-processed file generated from
all particles, for two polishing runs.
Best regards,
Takanori Nakane
On 2018/11/02 21:13, Huy Bui wrote:
> Dear Takanori,
>
> Thanks a lot. Just to be clear, for Bayesian polishing, I use the combined
> postprocessed star file for each data set separately?
> H
>
> On Fri, Nov 2, 2018, 3:09 PM Takanori Nakane <[log in to unmask]>
> wrote:
>
>> Hi,
>>
>> CTF refinement can be done on the combined dataset. In contrast,
>> you should run Bayesian Polishing separately because weighting for
>> each frame should be different.
>>
>> 1. Run Refine3D and Postprocessing using all particles from
>> dataset 1 and 2.
>> 2. Split run_data.star into two star files, one containing
>> particles from the dataset 1, the other from the dataset 2.
>> 3. Run Bayesian Polishing separately.
>> 4. Combine both.
>>
>> Best regards,
>>
>> Takanori Nakane
>>
>> On 2018/11/02 15:00, Huy Bui wrote:
>>> Dear all,
>>>
>>> I have 2 datasets of the same complex. The only difference between the
>> two
>>> datasets is that the dose per frame is 5.6e/A^2 in one and 4.2e/A^2.
>>>
>>> What is the best way to do it with Bayesian polishing?
>>> 1. I process the data separately using Bayesian polishing to produce the
>>> shiny particles and then combine the particles for CTFrefinement.
>>> 2. I combine the data and do Bayesian polishing the combined dataset.
>>>
>>> Best,
>>> Huy
>>>
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>>
>>
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