Dear Da,
In IMAGIC-4D you can perform the necessary camera correction!
(https://www.nature.com/articles/srep10317). It does it better than any
manufactures correction and improves the data significantly even when
performed after using the standard gain correction.
Cheers,
Marin
=====================================================
On 01/10/2018 15:36, Da Cui wrote:
> Hi all,
> The gain reference image for one dataset was missing by accident. In order to achieve a more accurate motioncor result, does anyone have idea about how to generate a gain reference image from the dataset (around 3k movies)?
> Thank you so much for your help!!!
> ---Da
>
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==============================================================
Prof Dr Ir Marin van Heel
Laboratório Nacional de Nanotecnologia - LNNano
CNPEM/LNNano, Campinas, Brazil
tel: +55-19-3518-2316
mobile +55-19-983455450 (current)
mobile +55-19-981809332
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Skype: Marin.van.Heel
email: marin.vanheel(A_T)gmail.com
marin.vanheel(A_T)lnnano.cnpem.br
and: mvh.office(A_T)gmail.com
--------------------------------------------------
Emeritus Professor of Cryo-EM Data Processing
Leiden University
Mobile NL: +31(0)652736618 (ALWAYS ACTIVE SMS)
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Emeritus Professor of Structural Biology
Imperial College London
Faculty of Natural Sciences
email: m.vanheel(A_T)imperial.ac.uk
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there is no guarantee that I will actually read each incoming email.
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