Hi,
>“_rlnMicrographShiftX” and “_rlnMicrographShiftY”
These are in pixels of the original (unbinned) movies.
I think you don't have to edit STAR files.
Specifying --mps (movie pixel size) and --cps (coordinate pixel size =
binned pixel size before
extraction) should do the trick.
Best regards,
Takanori Nakane
> Hello everyone-
>
> I am processing a dataset with Relion 3, and I realized that I have made
a c
> areless error. My data were collected in superresolution mode on a K2, but
> during motion correction I accidentally entered the non-superresolution,
bin
> ned pixel size (e.g. 1.0 instead of 0.5). I’ve now gotten to the point whe
> re I would like to do Bayesian polishing, but I would prefer not to have to
> re-align all of the movies. Instead, it would be great if I could edit
the
> star files which contain the trajectories so that they match the proper
pix
> el size.
>
> So my question is, what are the units of the “_rlnMicrographShiftX” and “
> _rlnMicrographShiftY”? Are they in pixels, or Angstroms? I am hoping th
> at simply editing these if they are in Angstroms, as well as the “_rlnMicro
> graphOriginalPixelSize” will be sufficient. If anyone else has dealt with
> this little mistake successfully, any comments would be appreciated : )
>
> Thanks,
> -Greg Alushin
>
>
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