We have a dataset in which most micrographs contain 200 - 300 particles, but a minority contain few or even no particles after 3D classification and selection. Rather than regrouping all the data, it's been my practice to gather the list of offending micrographs during an initial run of re-extraction with refined coordinates, then to delete the corresponding entries from the selected particles.star file with sed, and finally to run a fresh extraction with the reduced set of refined coordinates. Is this reasonable? When I do this, the number of extracted particles is reduced as I'd expect, but for some reason there are still warnings during extraction about micrographs with too few particles. Are these ignorable?
On a related note, I see from the Subsets tab in Subset Selection that there's new (or possibly in progress) work to facilitate screen based on various criteria. Would it be possible to include min / max number of particles per micrograph to such screens in the future?
Thanks!
-jh-
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