Hi,
You can do it in the particle extraction job.
But you have to calculate the center of mass of a mask yourself.
You can use the --com option of the relion_image_handler.
Best regards,
Takanori Nakane
On 2018/10/12 19:52, Oliver Clarke wrote:
> Hi,
>
> In the RELION multi-body paper, it is stated that:
>
> “...the relion_flex_analyse program can output subtracted images according to the iteratively refined relative orientations from a multi-body refinement. The resulting subtracted particle images can then be used in subsequent focused classifications and/or refinements. An additional advantage of this program over previously existing subtraction tools in relion is the centering at the projected centre-of-mass of the subtracted particle images, which allows the use of more meaningful priors and smaller box sizes.”
>
> Is this option (recentering and reboxing around the center of mass of a subregion) available anywhere else in Relion 3, as an independent job outside of the multi-body module? It would be a very handy option for existing focused refinements, or during signal subtraction.
>
> Cheers
> Oli
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