That's a good suggestion. You can do similar OR operations on binary masks
also in relion_mask_create.
S
> Hi Alex
>
> Its not really the solution for what you ask for, but is better than none.
>
> You can open all the binary masks in chimera and "vop max" them together.
> And then using relion_mask_create add a soft edge to the "consensus mask".
> This should let you make one mask that works for all the references and is
> hopefully better than a spherical mask. Aligning the references together
> so
> that you minimize the mask is desired if you do this.
>
> Best wishes
> Shintaro
>
> On Mon, Oct 1, 2018 at 5:52 PM Alex Cook <[log in to unmask]>
> wrote:
>
>> Hey all,
>>
>> I'm running a supervised (multi-reference) 3D classification in Relion
>> 3.0. Does anyone know if it is possible to provide solvent masks for
>> each of the references? To provide multiple references you give a
>> starfile with reference filenames under the rlnReferenceImage label.
>> Doing this for the masks (with the rlnSolventMaskName label) raises this
>> error: 'ERROR: invalid Spider file'.
>>
>> Has anyone managed to do this? Any insights are greatly appreciated!
>>
>> Cheers,
>> Alex
>>
>> PhD Student,
>> Birkbeck College
>>
>>
>>
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>
>
> --
> Yours Sincerely,
> Shintaro Aibara
>
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--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
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tel: +44 (0)1223 267061
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