Hi Takanori,
Will try that, thanks!
Xinchao
> On Oct 30, 2018, at 6:51 AM, Takanori Nakane <[log in to unmask]> wrote:
>
> Hi,
>
>> I was thinking maybe one could just rescale the particles during
>> extraction and then join the star files using relion3
>
> Yes. That is what we do.
>
> Example:
> dataset 1: 1.1 A / px
> dataset 2: 1.0 A / px
>
> Extract 200 px boxes from dataset 1. These are in 1.1 A / px and 220 * 1.1
> = 220 A wide.
> Extract 220 px boxes (= 220 A wide) from dataset 2 with down-sampling to
> 200 px.
> These are also in 220 * 1 / 200 = 1.1 A / px now.
> Join two star files, run Refine3D, CtfRefine and Refine3D again.
>
> In RELION, down-sampling is done by cropping in the reciprocal space.
>
> In the next version of RELION (not 3.0, but the next), we plan to enable
> refinement
> of particles with different pixel sizes (and with anisotropic magnification).
>
> Best regards,
>
> Takanori Nakane
>
>> Hi Sergry,
>> That’s useful to read through, I was thinking maybe one could just rescal
>> e the particles during extraction and then join the star files using
> relion3
>> . Has anybody tried this approach? Thanks
>> Xinchao
>> On Oct 30, 2018, at 12:47 AM, Sergey Nazarov
>> <[log in to unmask]<mailto:[log in to unmask]>> wrote:
>> Hi Xinchao,
>> Have a look at this thread
>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ccpem;1f8bb512.1602
>> and attached file (unfortunately, don't remember the author).
>> Best,
>> Sergey Nazarov
>> CIME, EPFL, Lausanne
>> вт, 30 окт. 2018 г. в 5:42, Xinchao <[log in to unmask]<mailto:xin
>> [log in to unmask]>>:
>> Hi all,
>> I have two data sets of the same sample that were collected at
>> slightly different pixel sizes (~5% difference), does anybody have a
>> good way of merging the two data sets?
>> Thanks,
>> Xinchao
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>> <Combining_data with different pixel sizes.pdf>
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