Hi,
> Do all the recent updates contain these same fixes or some do and some
> don't?
The latest version on the repository contains all fixes.
> It seems like B-factors got worse for 877139d9dd as I mentioned below,
> and then this particular fix needs to be looked at again?
Your B factors are unreliable even for early frames.
> Also, do you plan to continue working on the general fix to this, as we
> really would like to polish this data?
We keep investigating but cannot say anything about ETA.
Best regards,
Takanori Nakane
On 2018/10/25 19:20, Leonid Sazanov wrote:
> Hi, thanks for the info!
>
> Do all the recent updates contain these same fixes or some do and some
> don't?
>
> It seems like B-factors got worse for 877139d9dd as I mentioned below,
> and then this particular fix needs to be looked at again?
>
> Also, do you plan to continue working on the general fix to this, as we
> really would like to polish this data?
>
> Many thanks,
>
> Leonid
>
> Prof. Leonid Sazanov
> IST Austria
> Am Campus 1
> A-3400 Klosterneuburg
> Austria
>
> Phone: +43 2243 9000 3026
> E-mail: [log in to unmask]
>
> On 23/10/18 15:18, Takanori Nakane wrote:
>> Hi,
>>
>> Thank you very much for testing.
>>
>>> What exactly is the problem here, can you describe it briefly, please?
>> In the first stage of Bayesian Polishing, trajectories for each particle
>> and FCCs
>> for each movie are calculated. Next, FCCs are combined and B factors are
>> estimated.
>>
>> NaNs in trajectories and FCCs can lead to crash during B factor
>> estimation and/or Refine3D. Recent patches exclude movies containing
>> NaNs.
>> We don't know why and when NaNs are generated. One patch (294ce0d) fixes
>> one possible cause. These updates solved (well, work-around) at least
>> three cases, but seems not sufficient in your case.
>>
>> Best regards,
>>
>> Takanori Nakane
>>
>>> Dear Takanori,
>>> Tried the latest fix (877139d9dd)
>>> Still get the error after frame 9, and this time b-factors for the first
>>> 9 frames seem to make less sense than before, so it got worse in a way.
>>> What exactly is the problem here, can you describe it briefly, please?
>>> Thanks for your efforts!
>>> Here are new B-factors:
>>> # RELION; version 3.0-beta-2
>>> data_perframe_bfactors
>>> loop_
>>> _rlnMovieFrameNumber #1
>>> _rlnBfactorUsedForSharpening #2
>>> _rlnFittedInterceptGuinierPlot #3
>>> ?????????? 0???? -1.01236???? 0.003240
>>> ?????????? 1??? -11.50108???? 0.001786
>>> ?????????? 2??? -16.51618???? -0.02748
>>> ?????????? 3??? -22.32118???? -0.01753
>>> ?????????? 4??? -28.48895???? -0.00305
>>> ?????????? 5??? -31.38510???? -0.02080
>>> ?????????? 6??? -34.23007???? -0.01237
>>> ?????????? 7??? -40.68527???? 0.020047
>>> ?????????? 8??? -45.91777???? 0.041472
>>> ?????????? 9??? -49.76217???? 0.125882
>>> ????????? 10 -8.80873e+10???????? -inf
>>> ????????? 11 -8.80873e+10???????? -inf
>>> And here are previous on the same data:
>>> # RELION; version 3.0-beta-2
>>> data_perframe_bfactors
>>> loop_
>>> _rlnMovieFrameNumber #1
>>> _rlnBfactorUsedForSharpening #2
>>> _rlnFittedInterceptGuinierPlot #3
>>> ?????????? 0??? -11.23603???? 0.049748
>>> ?????????? 1???? -0.08144???? -0.21510
>>> ?????????? 2???? -0.08144???? -0.25988
>>> ?????????? 3???? -1.65827???? -0.30094
>>> ?????????? 4???? -6.20297???? -0.29457
>>> ?????????? 5??? -12.73199???? -0.27411
>>> ?????????? 6??? -18.13065???? -0.24068
>>> ?????????? 7??? -21.96097???? -0.23674
>>> ?????????? 8??? -26.33093???? -0.20546
>>> ?????????? 9??? -29.74517???? -0.11742
>>> ????????? 10 -8.80873e+10???????? -inf
>>> ????????? 11 -8.80873e+10???????? -inf
>>> Prof. Leonid Sazanov
>>> IST Austria
>>> Am Campus 1
>>> A-3400 Klosterneuburg
>>> Austria
>>> Phone: +43 2243 9000 3026
>>> E-mail: [log in to unmask]
>>> On 19/10/18 20:15, Takanori Nakane wrote:
>>>> Hi,
>>>> Jasenko made two more commits (0ad988f, 294ce0d) after I posted
>>>> here yesterday. Did you try that? If the problem persists,
>>>> we need more time to investigate. This is not an easy bug to fix.
>>>> Best regards,
>>>> Takanori Nakane
>>>> On 2018/10/19 17:37, Leonid Sazanov wrote:
>>>>> Dear all,
>>>>> We tried this fix, but still get the same error at the same frame.
>>>>> Please let us know what else can be tried.
>>>>> Many thanks,
>>>>> Leonid
>>>>> Prof. Leonid Sazanov
>>>>> IST Austria
>>>>> Am Campus 1
>>>>> A-3400 Klosterneuburg
>>>>> Austria
>>>>> Phone: +43 2243 9000 3026
>>>>> E-mail: [log in to unmask]
>>>>> Web: https://ist.ac.at/research/life-sciences/sazanov-group/
>>>>> On 17/10/2018 18:55, Takanori Nakane wrote:
>>>>>> Dear all,
>>>>>> Jasenko made a workaround for this issue.
>>>>>> This is the latest commit (d9686b9) on our public repository.
>>>>>> Please re-compile the program and 'continue' the job.
>>>>>> If the trajectories also contain NaNs, it might still produce
>>>>>> broken particles. If you notice it (i.e. Refine3D crashing),
>>>>>> please let us know.
>>>>>> Best regards,
>>>>>> Takanori Nakane
>>>>>>> Dear Liz,
>>>>>>> No, we did not.
>>>>>>> I really hope developers will answer something, since now there are
>>>>>>> at
>>>>>>> least three groups experiencing this problem.
>>>>>>> Best wishes,
>>>>>>> Leonid
>>>>>>> Prof. Leonid Sazanov
>>>>>>> IST Austria
>>>>>>> Am Campus 1
>>>>>>> A-3400 Klosterneuburg
>>>>>>> Austria
>>>>>>> Phone: +43 2243 9000 3026
>>>>>>> E-mail: [log in to unmask]
>>>>>>> Web: https://ist.ac.at/research/life-sciences/sazanov-group/
>>>>>>> On 13/10/2018 22:42, Liz Kellogg wrote:
>>>>>>>> Hi Leonid,
>>>>>>>> I'm experiencing the same issues. Did you ever get the issue
>>>>>>>> resolved?
>>>>>>>> Thanks
>>>>>>>> Liz
>>>>>>>> On Sat, Oct 13, 2018 at 10:42 AM Leonid Sazanov <[log in to unmask]
>>>>>>>> <mailto:[log in to unmask]>> wrote:
>>>>>>>> ???? Dear Takanori and Jasenko,
>>>>>>>> ???? We still have not resolved this problem (reported by Domen
>>>>>>>> below),
>>>>>>>> ???? which seems to happen to others as well.
>>>>>>>> ???? Does polishing normally work for K2 superresolution images if
>>>>>>>> they
>>>>>>>> ???? have binned 2X in motioncor2?
>>>>>>>> ???? It seems that if we increase memory available the errors
>>>>>>>> change but
>>>>>>>> ???? in the end it is always segmentation fault, and always on
>>>>>>>> frame 10.
>>>>>>>> ???? Now we used 250 GB memory and still have this error.
>>>>>>>> ???? Would be great to resolve it.
>>>>>>>> ???? Many thanks
>>>>>>>> ???? Best wishes,
>>>>>>>> ???? Leonid
>>>>>>>> ???? Hi,
>>>>>>>> ???? Thanks for your suggestion. I have checked the directories
>>>>>>>> but my
>>>>>>>> ???? FCC files are all of the same size as well (29344 bytes).
>>>>>>>> ???? Best,
>>>>>>>> ???? Domen
>>>>>>>> ???? On 29-Sep-18 0:48, Zhaoming Su wrote:
>>>>>>>> ????? >
>>>>>>>> ????? > Hi Takanori and Jasenko,
>>>>>>>> ????? >
>>>>>>>> ????? >
>>>>>>>> ????? > My FCC files have the same size.
>>>>>>>> ????? >
>>>>>>>> ????? >
>>>>>>>> ????? >
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103863_Data_10103657_10103658_20180910_1942_FCC_w0.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103863_Data_10103657_10103658_20180910_1942_FCC_w1.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103863_Data_10103667_10103668_20180910_1938_FCC_cc.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103863_Data_10103667_10103668_20180910_1938_FCC_w0.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103863_Data_10103667_10103668_20180910_1938_FCC_w1.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103571_10103572_20180910_1945_FCC_cc.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103571_10103572_20180910_1945_FCC_w0.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103571_10103572_20180910_1945_FCC_w1.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103579_10103580_20180910_1946_FCC_cc.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103579_10103580_20180910_1946_FCC_w0.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103579_10103580_20180910_1946_FCC_w1.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103589_10103590_20180910_1946_FCC_cc.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103589_10103590_20180910_1946_FCC_w0.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103589_10103590_20180910_1946_FCC_w1.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103603_10103604_20180910_1943_FCC_cc.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103603_10103604_20180910_1943_FCC_w0.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103603_10103604_20180910_1943_FCC_w1.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103612_10103613_20180910_1943_FCC_cc.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103612_10103613_20180910_1943_FCC_w0.mrc
>>>>>>>> ????? > -rw-r--r-- 1 suzm bd 13924 Sep 27 17:58
>>>>>>>> FoilHole_10103864_Data_10103612_10103613_20180910_1943_FCC_w1.mrc
>>>>>>>> ????? >
>>>>>>>> ????? >
>>>>>>>> ????? > Best,
>>>>>>>> ????? >
>>>>>>>> ????? >
>>>>>>>> ????? > Zhaoming Su, Ph.D.
>>>>>>>> ????? >
>>>>>>>> ????? > Research Scientist, Bioengineering
>>>>>>>> ????? >
>>>>>>>> ????? > Stanford University
>>>>>>>> ????? > From: Takanori Nakane <[log in to unmask]
>>>>>>>> ???? <mailto:[log in to unmask]>>
>>>>>>>> ????? > Sent: Friday, September 28, 2018 3:14:59 PM
>>>>>>>> ????? > To: Zhaoming Su; [log in to unmask]
>>>>>>>> <mailto:[log in to unmask]>
>>>>>>>> ????? > Cc: Jasenko Zivanov
>>>>>>>> ????? > Subject: Re: [ccpem] Bayesian Polishing b-factor fitting
>>>>>>>> error
>>>>>>>> ????? >
>>>>>>>> ????? > Hi Domen and Zhaoming,
>>>>>>>> ????? >
>>>>>>>> ????? > I forward comments from Jasenko Zivanov (CC-ed),
>>>>>>>> ????? > the developer of Bayesian Polishing.
>>>>>>>> ????? >
>>>>>>>> ????? > Please make sure all FCC files under the job directory
>>>>>>>> ????? > have the same size.
>>>>>>>> ????? >
>>>>>>>> ????? > > Could it be that some of the (thousands of) partial FCC
>>>>>>>> files
>>>>>>>> ???? (*_FCC_cc.mrc, *_FCC_w0/1.mrc) are damaged?
>>>>>>>> ????? > >
>>>>>>>> ????? > > Sjors changed the mrc-reader afterwards, so that it would
>>>>>>>> ???? refuse to load a corrupted image.
>>>>>>>> ????? > > That problem should be gone in the current version.
>>>>>>>> ????? > >
>>>>>>>> ????? > > I had observed a corrupted partial FCC file once myself.
>>>>>>>> ????? > > This could definitely introduce NaNs into the FCC
>>>>>>>> computation.
>>>>>>>> ????? > >
>>>>>>>> ????? > > The way to diagnose this would be to check if there are
>>>>>>>> ???? unusually small files in the output-directory.
>>>>>>>> ????? > > All of them should be the same size.
>>>>>>>> ????? >
>>>>>>>> ????? > Best regards,
>>>>>>>> ????? >
>>>>>>>> ????? > Takanori Nakane
>>>>>>>> ????? >
>>>>>>>> ????? > On 2018/09/28 17:34, Zhaoming Su wrote:
>>>>>>>> ????? > > Hi All,
>>>>>>>> ????? > >
>>>>>>>> ????? > > I have the exact problem on multiple data sets collected
>>>>>>>> as
>>>>>>>> ???? packed mrc from EPU, except they all fail at the second
>>>>>>>> frame...
>>>>>>>> ????? > >
>>>>>>>> ????? > > # RELION; version 3.0-beta-2
>>>>>>>> ????? > >
>>>>>>>> ????? > > data_perframe_bfactors
>>>>>>>> ????? > >
>>>>>>>> ????? > > loop_
>>>>>>>> ????? > > _rlnMovieFrameNumber #1
>>>>>>>> ????? > > _rlnBfactorUsedForSharpening #2
>>>>>>>> ????? > > _rlnFittedInterceptGuinierPlot #3
>>>>>>>> ????? > >? ? ? ? ? ? ?0? ? ?-0.04828 6.304424e-05
>>>>>>>> ????? > >? ? ? ? ? ? ?1 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?2 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?3 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?4 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?5 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?6 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?7 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?8 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?9 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 10 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 11 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 12 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 13 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 14 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 15 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 16 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 17 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 18 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 19 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 20 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 21 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 22 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 23 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? 24 -5.15980e+10? ? ? ? ?-inf
>>>>>>>> ????? > >
>>>>>>>> ????? > >
>>>>>>>> ????? > >
>>>>>>>> ????? > > Best,
>>>>>>>> ????? > >
>>>>>>>> ????? > >
>>>>>>>> ????? > > Zhaoming Su, Ph.D.
>>>>>>>> ????? > >
>>>>>>>> ????? > > Research Scientist, Bioengineering
>>>>>>>> ????? > >
>>>>>>>> ????? > > Stanford University
>>>>>>>> ????? > >
>>>>>>>> ????? > > ________________________________
>>>>>>>> ????? > > From: Collaborative Computational Project in Electron
>>>>>>>> ???? cryo-Microscopy <[log in to unmask]
>>>>>>>> <mailto:[log in to unmask]>>
>>>>>>>> ???? on behalf of Domen Kampjut <[log in to unmask]
>>>>>>>> ???? <mailto:[log in to unmask]>>
>>>>>>>> ????? > > Sent: Friday, September 28, 2018 7:45:40 AM
>>>>>>>> ????? > > To: [log in to unmask] <mailto:[log in to unmask]>
>>>>>>>> ????? > > Subject: [ccpem] Bayesian Polishing b-factor fitting
>> error
>>>>>>>> ????? > >
>>>>>>>> ????? > > Dear All,
>>>>>>>> ????? > >
>>>>>>>> ????? > > I keep running into an error when trying to polish
>>>>>>>> particles in
>>>>>>>> ????? > > relion3.0. Polishing job starts normally and estimated
>>>>>>>> per-particle
>>>>>>>> ????? > > motion for all micrographs look very reasonable judging
>>>>>>>> from
>>>>>>>> ???? the .eps
>>>>>>>> ????? > > files in the Micrographs subfolder. Estimating B-factors
>>>>>>>> fails
>>>>>>>> ???? after
>>>>>>>> ????? > > 10th frame though (see the bfactors.star output below)
>>>>>>>> and the
>>>>>>>> job
>>>>>>>> ????? > > terminates with segmantation fault. I only see this
>>>>>>>> error in
>>>>>>>> ???? the dataset
>>>>>>>> ????? > > collected with a K2 camera as packed .mrc movies using
>>>>>>>> EPU.
>>>>>>>> ???? Polishing of
>>>>>>>> ????? > > Falcon-III movies works fine. Also, if I limit the
>>>>>>>> polishing
>>>>>>>> ???? job to the
>>>>>>>> ????? > > first 9 frames the job completes normally. Please let me
>>>>>>>> know
>>>>>>>> ???? if anyone
>>>>>>>> ????? > > else observed the same error and what you would need to
>>>>>>>> ???? reproduce it.
>>>>>>>> ????? > >
>>>>>>>> ????? > > Many thanks and best wishes,
>>>>>>>> ????? > >
>>>>>>>> ????? > > Domen Kampjut
>>>>>>>> ????? > >
>>>>>>>> ????? > >
>>>>>>>> ????? > >
>>>>>>>> ????? > >
>>>>>>>> --------------------------------------------------------------
>>>>>>>> ????? > >
>>>>>>>> ????? > > data_perframe_bfactors
>>>>>>>> ????? > >
>>>>>>>> ????? > > loop_
>>>>>>>> ????? > > _rlnMovieFrameNumber #1
>>>>>>>> ????? > > _rlnBfactorUsedForSharpening #2
>>>>>>>> ????? > > _rlnFittedInterceptGuinierPlot #3
>>>>>>>> ????? > >? ? ? ? ? ? ? 0? ? -10.90545? ? ?0.048441
>>>>>>>> ????? > >? ? ? ? ? ? ? 1? ? ?-0.08144? ? ?-0.21434
>>>>>>>> ????? > >? ? ? ? ? ? ? 2? ? ?-0.08144? ? ?-0.26051
>>>>>>>> ????? > >? ? ? ? ? ? ? 3? ? ?-3.19811? ? ?-0.29255
>>>>>>>> ????? > >? ? ? ? ? ? ? 4? ? ?-5.69430? ? ?-0.29903
>>>>>>>> ????? > >? ? ? ? ? ? ? 5? ? -12.85827? ? ?-0.27141
>>>>>>>> ????? > >? ? ? ? ? ? ? 6? ? -19.60021? ? ?-0.24128
>>>>>>>> ????? > >? ? ? ? ? ? ? 7? ? -22.26604? ? ?-0.23126
>>>>>>>> ????? > >? ? ? ? ? ? ? 8? ? -28.06880? ? ?-0.19417
>>>>>>>> ????? > >? ? ? ? ? ? ? 9? ? -30.55454? ? ?-0.11258
>>>>>>>> ????? > >? ? ? ? ? ? ?10 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?11 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?12 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?13 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?14 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?15 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?16 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?17 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?18 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?19 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?20 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?21 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?22 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?23 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?24 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?25 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?26 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?27 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?28 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?29 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?30 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?31 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?32 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?33 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?34 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?35 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?36 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?37 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?38 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >? ? ? ? ? ? ?39 -8.80873e+10? ? ? ? ?-inf
>>>>>>>> ????? > >
>>>>>>>> ????? > >
>>>>>>>> ########################################################################
>>>>>>>>
>>>>>>>> ????? > >
>>>>>>>> ????? > > To unsubscribe from the CCPEM list, click the following
>>>>>>>> link:
>>>>>>>> ????? > >
>>>>>>>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
>>>>>>>> ????? > >
>>>>>>>> ????? > >
>>>>>>>> ########################################################################
>>>>>>>>
>>>>>>>> ????? > >
>>>>>>>> ????? > > To unsubscribe from the CCPEM list, click the following
>>>>>>>> link:
>>>>>>>> ????? > >
>>>>>>>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
>>>>>>>> ????? > >
>>>>>>>> ????? >
>>>>>>>> ????? >
>>>>>>>> ????? > To unsubscribe from the CCPEM list, click the following
>>>>>>>> link:
>>>>>>>> ????? >
>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
>>>>>>>> ???? To unsubscribe from the CCPEM list, click the following link:
>>>>>>>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
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>>>>>>>> ???? To unsubscribe from the CCPEM list, click the following link:
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>>>>>>> ########################################################################
>>>>>>>
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