Hi Da,
cisTEM contains two command line programs "sum_all_tif_files" and
"sum_all_mrc_files" which can be used to create a gain reference from
all the tif or mrc files in a given directory.
Cheers,
Tim
On 10/01/2018 02:51 PM, Craig Yoshioka wrote:
> Hi Da,
>
> I’ve had the same thing happen before (2000+ stacks), I just averaged all the data with a little bit of outlier removal.
>
> I believe Scipion also has a more disciplined way to generate normalization images.
>
>
>> On Oct 1, 2018, at 11:36 AM, Da Cui <[log in to unmask]> wrote:
>>
>> Hi all,
>> The gain reference image for one dataset was missing by accident. In order to achieve a more accurate motioncor result, does anyone have idea about how to generate a gain reference image from the dataset (around 3k movies)?
>> Thank you so much for your help!!!
>> ---Da
>>
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