Hello again
It could be a powder pattern aligned along one axis?
The cell I gave (actually orthorhombic) is one of the crystal forms of one of the components in your mixture – glycerol. You may have another form of glycerol. It is worth checking.
Colin
PS I think glycerol diffraction has been raised previously on CCP4bb
From: Sam Tang <[log in to unmask]>
Sent: 09 October 2018 15:02
To: Nave, Colin (DLSLtd,RAL,LSCI) <[log in to unmask]>
Cc: ccp4bb <[log in to unmask]>
Subject: Re: [ccp4bb] Weird diffraction pattern
Hello Colin
Although the unit cell dimensions from mosflm should be largely unreliable in this case, the software actually returned a P2 space group with a=24.6, b=7.5, c=69.5 where b is so short that it resembles a small molecule crystal.
Regards
Sam
Sam
On Tue, 9 Oct 2018 at 20:53, [log in to unmask]<mailto:[log in to unmask]> <[log in to unmask]<mailto:[log in to unmask]>> wrote:
Sam
Would this unit cell index some of the spots?
a = 7.00 ± 0.04 A, b = 9.96 ± 0.05 A, c = 6.29 ± 0.04 A.
Colin
From: CCP4 bulletin board <[log in to unmask]<mailto:[log in to unmask]>> On Behalf Of Sam Tang
Sent: 09 October 2018 12:13
To: ccp4bb <[log in to unmask]<mailto:[log in to unmask]>>
Subject: [ccp4bb] Weird diffraction pattern
Dear all
Hello. We recently shot a crystal (a protein with small molecule as ligand) at a synchrotron source and see a weird pattern. (https://drive.google.com/file/d/11bEtTJzKaAB5ZybezgN1cqBrckSRg2OV/view?usp=sharing)
Crystal was grown in Citric acid and ammonium sulfate, cryoprotected with glycerol.
At first we thought it was a protein crystal contaminated with salt but on second thought, the lowest resolution spot was at around 7 A, which doesn't make sense for a protein. So we would like to solicit your experience and perhaps someone may have encountered similar pattern before?
Many thanks.
Kind regards
Sam
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