Hi Dirk,
The micrographs are low-pass filtered and then displayed, which makes it
a lot easier to see the particles. You can use --low_pass in
image_handler to filter particles before displaying.
HTH,
Sjors
On 09/18/2018 08:46 AM, Dirk Kostrewa wrote:
>
> Dear RELION users,
>
> I'm working my way through the RELION 3.0beta tutorial and noticed
> that particles in micrographs are easily recognisable using the
> "Manual picking" and "Auto-picking" tasks. However, displaying
> micrographs and extracted particles from other tasks yield mainly
> grey-on-grey images that are hardly interpretable. Playing with the
> "Sigma contrast" doesn't really improve the images.
>
> I already found this page
> <https://www2.mrc-lmb.cam.ac.uk/relion/index.php?title=DisplayImages>
> describing the different image viewers in RELION. My questions are:
>
> Can the same settings as in particle picking be applied in other tasks
> for improved display of micrographs and particles?
>
> If not, could you please give me some hints for useful parameters of
> the relion_image_handler program for getting a good appearance of
> micrographs and particles?
>
> Best regards,
>
> Dirk.
>
> --
>
> **************************************************
> Dr. Dirk Kostrewa
> Gene Center Munich
> Department of Biochemistry, AG Hopfner
> Ludwig-Maximilians-Universität München
> Feodor-Lynen-Str. 25
> D-81377 Munich
> Germany
> Phone: +49-89-2180-76845
> Fax: +49-89-2180-76998
> E-mail: [log in to unmask]
> WWW: www.genzentrum.lmu.de
> **************************************************
>
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--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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