Thanks, Takanori!
Regards,
-jh-
--
John M. Heumann
Department of Molecular, Cellular, and Developmental Biology
347 UCB, University of Colorado
Boulder, CO 80309-0347
On 09/24/2018 04:20 AM, Takanori Nakane wrote:
> Hi,
>
> GCTF 1.18 changed a line from "CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]"
> to "CS[mm], HT[kV], ac, XMAG, DStep[um]" for unknown reasons.
>
> We updated the wrapper. Please update your local installation from our
> BitBucket.
>
> Best regards,
>
> Takanori Nakane
>
>> Does Relion 3.1Beta support Gctf version 1.18-B1 (the special phase plate
>> version)?
>> If I use V1.06, I get the expected outputs, but if I try to use V1.18-B1
>> with additional options
>> --cosearch_refine_ps 1 --refine_2d_T 1 --overlap 0.8 --do_EPA
>> --smooth_resL 20 --phase_shift_S 3
>> I wind up with warnings like "WARNING: skipping, since cannot get CTF
>> values for..."
>> Not surprisingly. this results in an empty micrographs_ctf.star file, and
>> no eps files or logfile.
>> The defocus and other values are all in the gctf*.out files, and seem to
>> be formatted identically to those in version 1.06.
>> Is the Gctf 1.18-B1 output format just different enough in some other way
>> to throw off parsing?
>>
>> Thanks!
>> -jh-
>>
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