Dear All,
I keep running into an error when trying to polish particles in
relion3.0. Polishing job starts normally and estimated per-particle
motion for all micrographs look very reasonable judging from the .eps
files in the Micrographs subfolder. Estimating B-factors fails after
10th frame though (see the bfactors.star output below) and the job
terminates with segmantation fault. I only see this error in the dataset
collected with a K2 camera as packed .mrc movies using EPU. Polishing of
Falcon-III movies works fine. Also, if I limit the polishing job to the
first 9 frames the job completes normally. Please let me know if anyone
else observed the same error and what you would need to reproduce it.
Many thanks and best wishes,
Domen Kampjut
--------------------------------------------------------------
data_perframe_bfactors
loop_
_rlnMovieFrameNumber #1
_rlnBfactorUsedForSharpening #2
_rlnFittedInterceptGuinierPlot #3
0 -10.90545 0.048441
1 -0.08144 -0.21434
2 -0.08144 -0.26051
3 -3.19811 -0.29255
4 -5.69430 -0.29903
5 -12.85827 -0.27141
6 -19.60021 -0.24128
7 -22.26604 -0.23126
8 -28.06880 -0.19417
9 -30.55454 -0.11258
10 -8.80873e+10 -inf
11 -8.80873e+10 -inf
12 -8.80873e+10 -inf
13 -8.80873e+10 -inf
14 -8.80873e+10 -inf
15 -8.80873e+10 -inf
16 -8.80873e+10 -inf
17 -8.80873e+10 -inf
18 -8.80873e+10 -inf
19 -8.80873e+10 -inf
20 -8.80873e+10 -inf
21 -8.80873e+10 -inf
22 -8.80873e+10 -inf
23 -8.80873e+10 -inf
24 -8.80873e+10 -inf
25 -8.80873e+10 -inf
26 -8.80873e+10 -inf
27 -8.80873e+10 -inf
28 -8.80873e+10 -inf
29 -8.80873e+10 -inf
30 -8.80873e+10 -inf
31 -8.80873e+10 -inf
32 -8.80873e+10 -inf
33 -8.80873e+10 -inf
34 -8.80873e+10 -inf
35 -8.80873e+10 -inf
36 -8.80873e+10 -inf
37 -8.80873e+10 -inf
38 -8.80873e+10 -inf
39 -8.80873e+10 -inf
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